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Primary endometrial epithelial cells cultured on hard plastic (i.e. polystyrene) dishes rapidly undergo de-differentiation (Zhou et al. , 2022) and, in the presence of serum, further lose physiological attributes (Ghosh and Sengupta, 1995) and epithelial barrier integrity, and have increased cellular senescence by the third cell passage (Arnold et... See more
WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research
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Human endometrial organoids can be used to answer questions about uterine/placental cross-talk during placentation, and will provide a system for studying the pathogenesis and treatment of common conditions affecting women, such as endometriosis and endometrial cancer.
Long-term, hormone-responsive organoid cultures of human endometrium in a chemically-defined medium
animal models are not capturing
why we need them
The lack of menstrual shedding and the immune differences in animal models of endometriosis limit the translational potential to human applications.
WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research
“Histologically, endometriotic lesions are quite diverse. Clement (2007) first emphasized the presence of both epithelial and stromal cell types, while Greaves et al. (2017) showed how this tissue recruits immune cells that create a chronic inflammatory environment. Forster et al. (2019) expanded on this by linking vascularization to lesion growth,... See more
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slaytta kısmını açıklamak için Endometriotic lesions are heterogeneous, comprising epithelial and stromal cells within an inflammatory microenvironment enriched with immune cells, blood vessels, and nerve fibers (Clement, 2007; Greaves et al., 2017; Forster et al., 2019; Panir et al., 2022).
rganoids are a promising innovative tool to investigate the mechanistic features of endometriotic aetiology and advance the identification of potential novel therapeutic targets to treat those whose lives are compromised by endometriosis.
WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research
During menstruation, the functional layer of the endometrium is shed, which is followed by reepithelialization, regeneration, and cellular proliferation during the proliferative phase. Differentiation and secretory transformation of the endometrial glands occurs during the subsequent secretory phase and, if pregnancy does not occur, the entire... See more
Self-renewing endometrial epithelial organoids of the human uterus
The organoids phenocopied physiological responses of endometrial epithelium to hormones, including increased cell proliferation under estrogen and maturation upon progesterone. Moreover, the human endometrial organoids replicated the menstrual cycle under hormonal treatment at both the morpho-histological and molecular levels. Together, we... See more
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Organoids are self-organising, genetically stable, 3D culture systems containing both progenitor/stem and differentiated cells that resemble the tissue of origin.
Long-term, hormone-responsive organoid cultures of human endometrium in a chemically-defined medium
animal models are not capturing
why we need them
what is an organoid