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zeynep sude
Primary endometrial epithelial cells cultured on hard plastic (i.e. polystyrene) dishes rapidly undergo de-differentiation (Zhou et al. , 2022) and, in the presence of serum, further lose physiological attributes (Ghosh and Sengupta, 1995) and epithelial barrier integrity, and have increased cellular senescence by the third cell passage (Arnold et... See more
WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research
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We identified that disease-modified macrophages exhibit increased expression of IGF-1 in an in vitro model of endometriosis-associated macrophages and confirmed expression by lesion-resident macrophages in mice and women. Concentrations of IGF-1 were elevated in peritoneal fluid from women with endometriosis and positively correlate with their pain... See more
Macrophage-derived insulin-like growth factor-1 is a key neurotrophic and nerve-sensitizing factor in pain associated with endometriosis
The organoids phenocopied physiological responses of endometrial epithelium to hormones, including increased cell proliferation under estrogen and maturation upon progesterone. Moreover, the human endometrial organoids replicated the menstrual cycle under hormonal treatment at both the morpho-histological and molecular levels. Together, we... See more
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Endometriosis remains one of the most poorly understood conditions due to its molecular, hormonal, cellular, and heterogeneric complexity, and an overall lack of funding for research into female-only diseases (Smith, 2023; Zondervan et al. , 2023).
WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research
Endometriosis affects an estimated 190 million women (and those assigned female at birth) worldwide, with a significant personal and societal burden due to its painful and fertility-related symptoms (Nnoaham et al. , 2011; Zondervan et al. , 2020)
WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research
“Histologically, endometriotic lesions are quite diverse. Clement (2007) first emphasized the presence of both epithelial and stromal cell types, while Greaves et al. (2017) showed how this tissue recruits immune cells that create a chronic inflammatory environment. Forster et al. (2019) expanded on this by linking vascularization to lesion growth,... See more
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slaytta kısmını açıklamak için Endometriotic lesions are heterogeneous, comprising epithelial and stromal cells within an inflammatory microenvironment enriched with immune cells, blood vessels, and nerve fibers (Clement, 2007; Greaves et al., 2017; Forster et al., 2019; Panir et al., 2022).
rganoids are a promising innovative tool to investigate the mechanistic features of endometriotic aetiology and advance the identification of potential novel therapeutic targets to treat those whose lives are compromised by endometriosis.
WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research
Organoids are self-organising, genetically stable, 3D culture systems containing both progenitor/stem and differentiated cells that resemble the tissue of origin.
Long-term, hormone-responsive organoid cultures of human endometrium in a chemically-defined medium
animal models are not capturing
why we need them
what is an organoid