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I’m sharing the following details that may be useful to you:
(1) Plasmid Full Name:
pTRE3GBi_EF1A_Tet3G_IRES_Puro-VHH hFBXW11-mCherry
or pTRE-BI-hGRAD
> Bacterial Resistance: Ampicillin.
> Addgene Link: https://www.addgene.org/207837/
(2) Reference Paper:
https://rupress.org/jcb/article/223/2/e202304030/276463/hGRAD-A-
... See moreMercer_Pub 248
The study describes the development of a continuous evolution system to engineer small degron tags that can be inserted into endogenous genes and selectively degrade proteins using specific small molecules.
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